{"id":229977,"date":"2020-10-05T19:11:01","date_gmt":"2020-10-05T19:11:01","guid":{"rendered":"https:\/\/equicord.com\/?page_id=229977"},"modified":"2022-07-18T09:30:22","modified_gmt":"2022-07-18T09:30:22","slug":"nuestra-tecnologia","status":"publish","type":"page","link":"https:\/\/equicord.com\/en\/our-technology\/","title":{"rendered":"Our technology"},"content":{"rendered":"

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Mesenchymal Stem Cells<\/h1>\n

Once injected into the animal, the cells are activated by the inflammatory environment present in the damaged tissues. After their activation, the cells secrete various cytokines that trigger an anti-inflammatory cascade that improves the clinical symptoms of the animal and promotes tissue regeneration
\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1″ _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb background_color=\u00bb#d6deec\u00bb use_background_color_gradient=\u00bbon\u00bb background_color_gradient_stops=\u00bb#ffffff 0%|#d6deec 100%\u00bb background_color_gradient_start=\u00bb#ffffff\u00bb background_color_gradient_end=\u00bb#d6deec\u00bb custom_padding=\u00bb||55px|||\u00bb bottom_divider_color=\u00bbrgba(0,0,0,0)\u00bb bottom_divider_height=\u00bb24px\u00bb bottom_divider_repeat=\u00bb0x\u00bb bottom_divider_flip=\u00bbhorizontal\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row column_structure=\u00bb2_5,3_5″ _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb custom_margin=\u00bb|auto|47px|auto||\u00bb custom_margin_tablet=\u00bb-35px||||false|false\u00bb custom_margin_phone=\u00bb\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb custom_padding=\u00bb||0px|||\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb2_5″ _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb header_2_font_size=\u00bb44px\u00bb custom_margin_tablet=\u00bb||-20px||false|false\u00bb custom_margin_phone=\u00bb||-16px||false|false\u00bb custom_margin_last_edited=\u00bbon|desktop\u00bb custom_padding=\u00bb55px||0px|||\u00bb header_2_font_size_tablet=\u00bb\u00bb header_2_font_size_phone=\u00bb31px\u00bb header_2_font_size_last_edited=\u00bbon|phone\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n

What are Mesenchymal Stem Cells?<\/h2>\n

 
\n[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00bb3_5″ _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb header_2_font_size=\u00bb48px\u00bb custom_padding=\u00bb65px|||||\u00bb custom_padding_tablet=\u00bb0px||||false|false\u00bb custom_padding_phone=\u00bb0px||||false|false\u00bb custom_padding_last_edited=\u00bbon|desktop\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n

Mesenchymal stem cells are multipotential cells with the ability to differentiate into various tissues such as osteocytes (bone cells) and chondrocytes (cartilage cells).<\/p>\n

Mesenchymal stem cells are present in a multitude of tissues such as adipose tissue, bone marrow, umbilical cord, etc. Mesenchymal cells are responsible for the repair and regeneration of tissues in the body.<\/p>\n

Thanks to biotechnology, these cells can be isolated and cultured in vitro in order to be used in other patients as regenerative medicine treatments.<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1″ _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row column_structure=\u00bb2_5,3_5″ _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb custom_margin=\u00bb|auto|47px|auto||\u00bb custom_margin_tablet=\u00bb-35px||||false|false\u00bb custom_margin_phone=\u00bb\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb custom_padding=\u00bb||0px|||\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb2_5″ _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb header_2_font_size=\u00bb44px\u00bb custom_margin=\u00bb3vw||||false|false\u00bb custom_margin_tablet=\u00bb||||false|false\u00bb custom_margin_phone=\u00bb||-16px||false|false\u00bb custom_margin_last_edited=\u00bbon|phone\u00bb custom_padding=\u00bb||0px||false|false\u00bb header_2_font_size_tablet=\u00bb\u00bb header_2_font_size_phone=\u00bb31px\u00bb header_2_font_size_last_edited=\u00bbon|phone\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n

Umbilical cord use advantages\u00a0<\/span><\/h2>\n

[\/et_pb_text][et_pb_text _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb header_2_font_size=\u00bb48px\u00bb custom_margin=\u00bb0vw||||false|false\u00bb custom_margin_tablet=\u00bb3vw||||false|false\u00bb custom_margin_phone=\u00bb5vw||||false|false\u00bb custom_margin_last_edited=\u00bbon|desktop\u00bb custom_padding=\u00bb||||false|false\u00bb custom_padding_tablet=\u00bb0px||||false|false\u00bb custom_padding_phone=\u00bb0px||||false|false\u00bb custom_padding_last_edited=\u00bbon|desktop\u00bb global_colors_info=\u00bb{}\u00bb]Las c\u00e9lulas madre de cord\u00f3n umbilical presentan importantes ventajas que las hacen la fuente ideal para tratamientos de terapia celular.
\nGracias a sus singulares caracter\u00edsticas las c\u00e9lulas de cord\u00f3n umbilical permiten obtener un producto de mayor calidad, seguridad y eficacia a un menor coste de fabricaci\u00f3n.[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00bb3_5″ _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_accordion open_toggle_text_color=\u00bb#24519b\u00bb open_toggle_background_color=\u00bbrgba(214,222,236,0.35)\u00bb closed_toggle_background_color=\u00bb#d6deec\u00bb icon_color=\u00bb#24519b\u00bb _builder_version=\u00bb4.17.6″ _module_preset=\u00bbdefault\u00bb toggle_text_color=\u00bb#24519b\u00bb background_enable_color=\u00bboff\u00bb custom_margin=\u00bb3vw||||false|false\u00bb custom_margin_tablet=\u00bb\u00bb custom_margin_phone=\u00bb\u00bb custom_margin_last_edited=\u00bbon|desktop\u00bb border_radii=\u00bboff|20px|4px|20px|4px\u00bb border_width_all=\u00bb0px\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_accordion_item title=\u00bbExtracci\u00f3n no invasiva\u00bb open=\u00bbon\u00bb _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb]La extracci\u00f3n de MSC de cord\u00f3n umbilical (tanto de tejido como de sangre) se produce una vez el animal ha nacido sin manipulaci\u00f3n o intervenci\u00f3n en el proceso natural de parto del animal, siendo una extracci\u00f3n de tejido no invasiva. La extracci\u00f3n de c\u00e9lulas de un tejido por un proceso no invasivo evita someter al animal a una intervenci\u00f3n dolorosa evitando riesgos asociados a la extracci\u00f3n del tejido como efectos de la sedaci\u00f3n o cicatrizaci\u00f3n y estando en l\u00ednea con m\u00e1ximo el bienestar animal.[\/et_pb_accordion_item][et_pb_accordion_item title=\u00bb100% Bienestar animal\u00bb open=\u00bboff\u00bb _builder_version=\u00bb4.17.6″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb]Los cordones umbilicales utilizados en la fabricaci\u00f3n de nuestros productos provienen exclusivamente de yeguadas de alto nivel, estas deben tener instalaciones adecuadas para la gestaci\u00f3n, parto y lactancia de nuestros donantes, un veterinario permanente y un programa sanitario que garantice la m\u00e1xima salud y bienestar de nuestros potros. Gracias a nuestro proceso no invasivo, la obtenci\u00f3n de las c\u00e9lulas est\u00e1 100% en l\u00ednea con los m\u00e1ximos est\u00e1ndares de bienestar animal. Tras el parto, tanto la madre como el potro son seguidos por un veterinario que vela por su adecuado estado de salud y desarrollo. Una vez que los cordones han sido donados nuestros potros contin\u00faan su crecimiento normal junto con sus madres.[\/et_pb_accordion_item][et_pb_accordion_item title=\u00bbEficiencia en el aislamiento celular\u00bb _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb open=\u00bboff\u00bb]<\/p>\n

The number of mesenchymal stem cells that can be obtained from invasive sources is very limited. Only between 0.001% and 0.01% of mononuclear cells are considered MSC after the extraction of peripheral blood or bone marrow, while you can only find 5,000 cells for each gram of adipose tissue. In contrast, the number of cells in umbilical cord tissue (Wharton's jelly) is 50,000 per centimeter. In addition to the higher number of cells per gram of tissue, umbilical cord MSCs have a higher proliferation capacity compared to other tissue sources (Kalaszcynska et al., 2015). This makes the manufacturing more efficient.<\/p>\n

[\/et_pb_accordion_item][et_pb_accordion_item title=\u00bbEstado inmunoprivilegiado\u00bb _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb open=\u00bboff\u00bb]<\/p>\n

The ability to modulate immunological responses rank umbilical cord MSCs as an important compatible stem cell type for therapeutic applications in an allogeneic setting. The mechanisms of immune privilege are still being investigated; however, low MHC-I level and absence of MHC-II expression protect them from NK-mediated lysis.\n
\nIn addition, recent studies in horses demonstrate that in MSCs from conventional sources (Bone Marrow) a great heterogeneity in the expression of MHC-II surface markers exists (range 0\u201398% positive) (Schnabel, 2013). In addition, a study from the same author (Schanabel et al., 2014) showed that the incidence of positiveness of MHC-II in bone marrow samples is about 85%. However, in our experience in more than 100 donors of Umbilical Cord MSC that has been characterised, all of them were strongly negative to MHC-II (<1%)<\/p>\n

[\/et_pb_accordion_item][et_pb_accordion_item title=\u00bbBajas duplicaciones celulares\u00bb _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb open=\u00bboff\u00bb]<\/p>\n

Thanks to working with a tissue as rich in stem cells as the umbilical cord and the large anatomical size of an equine umbilical cord (~1kg), the number of stem cells obtained from a single umbilical cord is extremely higher than the one obtained from other sources.\nThis higher number of cells in pass zero (P0) allows the expansion of the cells efficiently without the need to increase the cellular duplications in excess. It is well known that as the cells are duplicated in culture, they lose differentiation capacity, immunomodulation and therapeutic actions (Crisostomo et al., 2006).<\/p>\n

[\/et_pb_accordion_item][et_pb_accordion_item title=\u00bbSeguridad\u00bb _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb open=\u00bboff\u00bb]Las c\u00e9lulas madre de cord\u00f3n umbilical son consideradas m\u00e1s seguras que las c\u00e9lulas procedentes de otros tejidos por diferentes razones. Por un lado, siendo un tejido recogido en el momento del nacimiento del animal las c\u00e9lulas donantes no est\u00e1n expuestas al ambiente del donante ni a otros agentes infecciones que puedan afectar al donante, reduciendo significativamente el riesgo de infecciones con virus extra\u00f1os (Nagamura-Inoue et al., 2014). Por otro lado las c\u00e9lulas no est\u00e1n expuestas a cambios derivados de la edad del donante o al ambiente, por lo que el riesgo de alteraciones gen\u00e9ticas y cromos\u00f3micas es mucho menor que con fuentes de c\u00e9lulas madre adultas.[\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1″ prev_background_color=\u00bb#ffffff\u00bb next_background_color=\u00bb#ffffff\u00bb _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb use_background_color_gradient=\u00bbon\u00bb background_color_gradient_direction=\u00bb108deg\u00bb background_color_gradient_stops=\u00bb#24519b 0%|#6eabd3 100%\u00bb background_color_gradient_start=\u00bb#24519b\u00bb background_color_gradient_end=\u00bb#6eabd3″ custom_margin=\u00bb-22px|||||\u00bb custom_padding=\u00bb||136px|||\u00bb top_divider_style=\u00bbramp\u00bb top_divider_height=\u00bb38px\u00bb top_divider_flip=\u00bbhorizontal\u00bb bottom_divider_style=\u00bbarrow2″ bottom_divider_height=\u00bb86px\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb custom_margin=\u00bb64px|auto||auto||\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4″ _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.16″ _module_preset=\u00bbdefault\u00bb header_2_text_color=\u00bb#ffffff\u00bb header_2_font_size=\u00bb37px\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n

Mechanism of action of MSCs<\/h2>\n

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The mechanism of action (MoA) of mesenchymal stem cells is complex, multifactorial and not fully understood. Initially it was thought that its efficacy lay in its inherent capacity for differentiation and adhesion to tissues, however, it is now known that this capacity in vivo<\/em> is very limited.<\/p>\n

Today it has been determined that the mechanism of action of MSCs is based mainly on the paracrine capacity and on the secretion of immunomodulatory factors that act on cells and tissues, generating an anti-inflammatory and regenerating environment.<\/p>\n

Once injected into the animal, the cells are activated by the inflammatory environment present in the damaged tissues. After their activation, the cells secrete various cytokines that trigger an anti-inflammatory cascade that improves the clinical symptoms of the animal and promotes tissue regeneration<\/p>\n

At EquiCord we have conducted in vitro<\/em> studies demonstrating the immunomodulatory capabilities of EUC-MSCs. This ability gives it a great local anti-inflammatory effect, which translates into a significant reduction in lameness and pain.<\/p>\n

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